It is widely used chromatography procedure among various instrumental techniques our philosophy of HPLC method development is based on several consideration. There exists today a really better practical understanding of chromatographic separation which is varies with sample & experimental condition strategy should based on as many experimental runs as are really required to achieve desire final result. Here by HPLC method development step strategy based on review some basics HPLC separation & more helpful facts that can ensure that method development start out in right direction. All critical steps in method development will be summarized and a sequence of events that required to develop the method efficiently will be proposed. This scheme described in this review will usually work in either situation.
Loading....